24-Hour Biological Indicator, 50 Tests — Autoclave Steam Spore Test

CliniEco 24-hour biological indicator, 50-pack — five self-contained indicators

CliniEco Medical Biological Indicator — 24-Hour Self-Contained, 50-Pack

$109.99 CAD

$2.20 CAD each

In stock

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CliniEco Medical Biological Indicator — 24-Hour Self-Contained, 50-Pack

Daily biological monitoring for Canadian dental clinics — 24-hour results at about $2.20 per test

In-office monitoring — incubate and read on site; no need to send vials to an outside lab.

G. stearothermophilus ATCC 7953 · ISO 11138-3

Daily spore testing — 24-hour results

$109.99 for 50 self-contained biological indicators — about $2.20 per test. Each vial contains Geobacillus stearothermophilus ATCC 7953 spores (≥1.0 × 10⁶ CFU per vial, typical 1.0–1.2 × 10⁶, as stated on each lot CoA), the standard challenge organism for steam sterilization, in a self-contained vial with growth medium for a rapid visual readout in 24 hours instead of 48. In Ontario, the RCDSO requires a biological indicator for each sterilizer on every day that it is used — daily monitoring, not weekly. The weekly baseline is the CDC / AAMI ST79 standard in most US states. Each monitoring day uses two indicators — one test plus one unexposed positive control — so a 50-pack gives about 25 monitoring days.

🛡️ Where Your BIs Come From — Traceability You Can Verify

🏭 Sourced from Certified Manufacturers

Our BIs are manufactured by ISO 13485-certified facilities — the same supply chain used by established sterilization brands. Full factory documentation available on request.

🧪 Standard Challenge Organism

Geobacillus stearothermophilus ATCC 7953 — the internationally accepted steam sterilization challenge organism, ≥1.0 × 10⁶ CFU per vial (typical 1.0–1.2 × 10⁶), verified per ISO 11138-1.

📋 Batch Traceability

Every pack carries lot-level traceability. Batch D-value and spore-count documentation available — ask us for the certificate of analysis for your lot.

🇨🇦 Canadian Import & Distribution

Imported and distributed by CliniEco Medical (MDEL #35334) — with English/French labeling and Toronto-based support.

📦 Importer & Regulatory Information

Imported and distributed by: CliniEco Medical (Ecology Source New Energy Technology Co., Ltd.)
255 Duncan Mill Road, Suite 512, Toronto, ON M3B 3H9, Canada
Storage: 15–30°C, away from direct sunlight and moisture. Shelf life: 24 months from manufacture; EXP printed on every vial. For professional use only. Do not use if damaged or expired.
Conforms to EN ISO 11138-3 (moist heat) and EN ISO 11138-1 — third-party test report XDX522601161208BST (BST Testing, EN ISO 11138-1:2017 and -3:2017) available on request. For steam sterilization only — not for dry heat, chemical vapour, ethylene oxide (EO) or other low-temperature processes.

Have a question about your own facility? Send it in and you will get a written answer specific to your setup, with the regulation or standard it is based on cited. Ask your compliance question.

✅ ISO 11138-3 Compliant ✅ ATCC 7953 Challenge Organism ✅ ISO 13485 Certified Factory ✅ Batch Traceability

When the 50-Pack Fits Better Than the 25 or the 100

A test day takes two indicators from the same lot — the vial that runs through the cycle and the unexposed control that must turn yellow for the run to be valid. Work out the year as test days multiplied by two, then pick the box that lands closest to it without leaving you short mid-quarter.

Test days a year	Indicators a year	50-packs a year	Cost in 50-packs, $109.99 each	The same year bought as 25-packs, $69.99 each
25	50	1	$109.99	2 boxes — $139.98
30	60	2	$219.98	3 boxes — $209.97
35	70	2	$219.98	3 boxes — $209.97
40	80	2	$219.98	4 boxes — $279.96
49	98	2	$219.98	4 boxes — $279.96

At $2.20 an indicator the 50-pack sits between the entry box and the yearly box. Choose it when your test calendar is real but not daily, or when you want the ordering rhythm to be quarterly instead of monthly.

Two situations this box is built for

Two sterilizers on alternating days. Each unit is tested on the days it actually runs, so the test days roughly double and the box empties at twice the pace. Buying two 25-packs a month for that rhythm costs more than one 50-pack a quarter.
A two-month to three-month restock cycle. A practice that runs two or three days a week gets about two to three months of cover from this box, which means four deliveries a year and a single lot number per quarter — easier to file and easier to trace back if a lot is ever questioned.
One sterilizer plus a backup unit. If the backup is run only on weeks when the main unit is serviced, the extra test days land in bursts; the 50-pack absorbs the burst without a special order.

When the 50-pack is the wrong box

More than 49 test days a year: step to the 100-pack at $1.89 an indicator, because the gap between $2.20 and $1.89 compounds across a year.
Under 25 test days a year, or a new routine you have not yet proven: the 25-pack at $99 covers twelve test days and keeps the exposure small.
Storage that runs warm or damp: indicator vials belong in a dry place at room temperature, and a box that sits for a year in a utility room is a risk you do not need to take for a few dollars a box.

What one box covers, week by week

Fifty indicators is 25 test days. At five test days a week that is five weeks; at three days a week, about eight weeks; at two days a week, about twelve. Write your own test days a week into that line before ordering — if the answer is over fourteen weeks, the box will outlast your patience before it outlasts the shelf, and the 25-pack is the better rhythm.

The same year, priced against the neighbours

Test days a year	Indicators	Bought as 50-packs	Bought as 25-packs	Bought as 100-packs
25	50	1 box — $109.99	2 boxes — $139.98	1 box — $189.00
40	80	2 boxes — $219.98	4 boxes — $279.96	1 box — $189.00

That last row is the honest one: at 80 indicators a year a single 100-pack is cheaper than two 50-packs, but it leaves twenty vials on the shelf. If your test days are steady and the lot date allows it, the larger box wins on price; if the routine is still settling, pay the small premium and keep the shelf light.

How to Read Your 24-Hour Result

Negative — pass	The medium stays purple after 24 hours at 56–60 °C (58 ± 2 °C): no spore growth. Release the load.
Positive — fail	The medium turns yellow: spores survived the cycle. Re-sterilize everything in that load and re-test before releasing instruments.
Incubation	56–60 °C (58 ± 2 °C) dry-block incubator; read at 24 hours (this indicator is rated for a 24-hour readout, not 48 hours).
Positive control	Run one unexposed indicator from the same lot as a control on each day you test; it must turn yellow. If the control does not grow, the test run is not valid.
Reading is visual	No reader or UV lamp is needed.
Source	Manufacturer's instructions for use supplied with each lot.

Product Specifications

Brand	CliniEco Medical
Organism	Geobacillus stearothermophilus ATCC 7953 — ≥1.0 × 10⁶ CFU per vial (typical 1.0–1.2 × 10⁶)
Readout	24-hour rapid visual readout (color change)
Cycles	121°C gravity & 132–135°C vacuum-assisted steam sterilization
Format	Self-contained vial with growth medium — no transfer steps
Pack Size	50 indicators per pack; monitoring log template included
Frequency	Daily per sterilizer in Ontario (RCDSO); at least weekly in most US states (CDC / AAMI ST79)
D-value / Z-value	Reported for every lot on the CoA — current lot: 2.0 min at 121 °C, Z-value 11.3 °C
Distribution	CliniEco MDEL #35334

Certificate of analysis for the 24-hour steam biological indicator (lot sample)

Sample CoA from a production lot — the certificate issued with your shipment carries your lot number and the manufacturer's details.

Features

✓ G. stearothermophilus ATCC 7953 — the accepted steam sterilization challenge organism
✓ 24-hour result — results a day sooner than 48-hour vials
✓ Self-contained (SCBI) — ampoule and medium in one vial
✓ Monitoring record log template included — supports RCDSO daily documentation and CDC / AAMI ST79

Compliance

RCDSO (Ontario): a biological indicator for each sterilizer on every day that it is used — daily, per RCDSO IPAC standard 03/24_5542
CDC and AAMI ST79: spore test at least weekly per sterilizer (US baseline)
Test organism: Geobacillus stearothermophilus ATCC 7953 (steam)
Distributed by CliniEco Medical under MDEL #35334
Manufactured to EN ISO 11138-3 and EN ISO 11138-1 — documentation available on request

Hardware is sold separately — buy only what you need

We do not bundle hardware into a plan. Prices:

24-Hour Dry-Block Incubator — $379
Rapid Reader, 4-well fluorescence — $1,499 (3-hour results)

Both include free printable record sheets. No lock-in — our BIs work in any standard 56–60 °C (58 ± 2 °C) incubator.

Start BI Plan — Reserve Your Free Device →

CliniEco Service Promise

Free shipping on this 50-pack — anywhere in Canada
Ships from our next production lot — dispatch date confirmed by email within one business day
Canadian-owned company
Batch documentation available — CoA, D-value and spore count on request

Wholesale Pricing

Volume pricing for clinics, dental practices, LTC homes and distributors
Free shipping on this item — anywhere in Canada

Why Buy CliniEco?

24-hour results at about $2.20 per test — 28% below our 25-pack unit price
Compliance built in — the monitoring record log template supports RCDSO daily documentation and CDC / AAMI ST79 expectations
Complete reprocessing line — pairs with CliniEco pouches, rolls, tape and cleaning solution
Full traceability — factory documentation, batch CoA, Canadian import

Frequently Asked Questions

How often should we run a spore test?

It depends on the jurisdiction. In Ontario, the RCDSO requires a biological indicator for each sterilizer on every day that it is used — daily monitoring. In most US states, the CDC and AAMI ST79 baseline is at least weekly. One 50-pack covers about one month of daily testing for one sterilizer.

How do I read the result?

After the cycle, activate the vial per the included instructions and incubate; read at 24 hours. No color change means no surviving spores (pass); a color change means failure — quarantine the load and re-test the sterilizer.

What organism is used, and why?

Geobacillus stearothermophilus ATCC 7953 — the standard biological challenge for steam because of its high heat resistance. A compact dry-block incubator is available on request.

Can I see the factory documentation?

Yes — we provide the test reports behind the standards listed above and batch certificates of analysis on request. Email alina@clinieco.ca.

Does it work with my autoclave cycle type?

This item is used with standard steam sterilization cycles; confirm the specific cycle against the manufacturer's instructions supplied with each lot.

How should it be stored, and how long does it keep?

Store between 15–30 °C at 30–65% relative humidity, keep away from sterilants, direct sunlight and UV, and do not crush the packaging. Shelf life is 24 months from the date of manufacture.

How often does a Canadian clinic need to run it?

In Ontario, the RCDSO requires a biological indicator for each sterilizer on every day that it is used — daily monitoring, not week.

Which standard does it meet?

Manufactured and tested to EN ISO 11138-1 and EN ISO 11138-3, in ISO 13485-certified facilities.

How long does a 24-hour readout biological indicator take to incubate?

This is a self-contained 24-hour test: after the cycle the indicator is activated and incubated, and the readout is available at the time stated in the manufacturer's instructions for use — around 24 hours. Keep the activated indicator in the incubator for the full period before reading it, and read the result against the control.

Is a 24-hour biological indicator acceptable for RCDSO daily monitoring?

The requirement is about how often you test, not how fast the indicator reads. In Ontario the RCDSO expects a biological indicator for each sterilizer on every day the sterilizer is used — daily monitoring, not weekly — placed in a process challenge device and run for each type of cycle used that day. The weekly interval is the CDC / AAMI ST79 baseline in most US states. A 24-hour readout fits a daily schedule; where same-shift release is needed, a rapid-readout indicator is used instead.

How is this different from the 3-hour indicator and the 5-pack trial?

All three are biological indicators with the same pass-or-fail meaning; the difference is the readout time and the pack size. The 3-hour unit is a fluorescence test for same-shift release, this 24-hour unit suits a next-day schedule, and the 5-pack trial is a small pack to evaluate the routine before committing to a working size.

How many indicators does one test need, and how long should records be kept?

One indicator per sterilizer for each day it is used, plus a control. File the result in a log that matches your facility's records-retention policy, and note the sterilizer, the cycle type and the date alongside it.

CliniEco Medical Biological Indicator — 24-Hour Self-Contained, 50-Pack

CliniEco Medical Biological Indicator — 24-Hour Self-Contained, 50-Pack

Daily spore testing — 24-hour results