CliniEco Medical Biological Indicator — 24-Hour Self-Contained, 100-Pack
Specifications
| Type | Self-contained biological indicator |
|---|---|
| Readout time | 24 hours |
| Pack quantity | 100 tests |
| Use | Steam autoclave sterilization monitoring |
| Result indication | Colour change |
| SKU | CLI-STER-BI-100 |
| Brand | CliniEco Medical |
Pricing
| Order | Unit price |
|---|---|
| 1 case | $189.00 CAD |
| 2–4 cases | Request quote |
| 5+ cases | Request quote |
Read the full product guide 23 sections · specs, use, compliance
CliniEco Medical Biological Indicator — 24-Hour Self-Contained, 100-Pack
How many monitoring days does a 100-vial box actually cover?
About fifty, because each monitoring day takes two vials rather than one: the exposed test vial from the load, plus an unexposed positive control drawn from the same lot. On a single sterilizer tested on every day it is used, that is roughly ten weeks of use-days; two units on the same schedule halve it. The arithmetic is the reason the 100-pack exists as a separate box rather than a multiple of the 50-vial box — a two-unit room that tests daily works through 100 vials in five to six weeks, and a light single-unit schedule is better served by the 25-vial box.
Which control vial does each test day need, and what happens when it fails?
An unexposed vial of the same lot, incubated alongside the test vial and never put through a cycle. Its job is to prove the conditions were right: if the control does not turn yellow, the incubation or the medium failed and the day's result cannot be read as a pass — the load is not released on that basis. Purple on the test vial with yellow on the control is the passing pattern; yellow on the test vial means the spores survived and everything in the load goes back through a cycle and a repeat test.
How does daily testing in Ontario compare with the weekly US baseline when sizing a 100-pack?
The frequency, not the product. Ontario dental guidance requires a biological indicator for each sterilizer on every day it is used, so a 100-pack is consumed at two vials a day rather than two a week. The weekly baseline most US states work to — the CDC and AAMI ST79 floor — would stretch the same box across most of a year. A clinic that follows the Ontario daily rule and orders on the weekly assumption runs out mid-quarter, which is the sizing error this box is meant to prevent.
| Item | As listed on this page |
|---|---|
| Indicators per box | 100 self-contained vials |
| Challenge organism | Geobacillus stearothermophilus ATCC 7953 |
| Spore load | ≥1.0 × 10⁶ CFU per vial, typically 1.0–1.2 × 10⁶ as stated on each lot certificate |
| Readout | 24-hour visual colour change, no reader or UV lamp |
| Incubation | 56–60 °C dry-block incubator |
| Cycles covered | 121 °C gravity-displacement and 132–135 °C vacuum-assisted steam |
| Standard | ISO 11138-1 and ISO 11138-3 |
| Monitoring days | About 50, at two vials (test plus positive control) per day |
| Frequency reference | Daily per sterilizer per day of use in Ontario; weekly as the CDC and AAMI ST79 floor |
| Distribution | CliniEco Medical, MDEL #35334 |
The box only counts if the result is written down: the printable spore test log sheet carries date, sterilizer, lot, control result and test result, and the same page holds the sterilization log generator for the cycle record behind it. Questions about what to retain, and for how long, go to the compliance documentation desk.
CliniEco Medical distributes this indicator in Canada under MDEL #35334.
Daily biological monitoring for Canadian dental clinics — 24-hour results, with the per-test figure taken from the box amount on this page
In-office monitoring — incubate and read on site; no need to send vials to an outside lab.
G. stearothermophilus ATCC 7953 · ISO 11138-3
Daily spore testing — 24-hour results
100 self-contained biological indicators in the box, priced on this page — the per-test figure is that amount divided by a hundred. Each vial contains Geobacillus stearothermophilus ATCC 7953 spores (≥1.0 × 106 CFU per vial, typical 1.0–1.2 × 106, as stated on each lot CoA), the standard challenge organism for steam sterilization, in a self-contained vial with growth medium for a rapid visual readout in 24 hours instead of 48. In Ontario, the RCDSO requires a biological indicator for each sterilizer on every day that it is used — daily monitoring, not weekly. The weekly baseline is the CDC / AAMI ST79 standard in most US states. Each monitoring day uses two indicators — one test plus one unexposed positive control — so a 100-pack gives about 50 monitoring days.
🛡️ Where Your BIs Come From — Traceability You Can Verify
🏭 Factory Documentation on Request
Every lot ships with full manufacturer documentation available on request — the same supply chain used by established sterilization brands.
🧪 Standard Challenge Organism
Geobacillus stearothermophilus ATCC 7953 — the internationally accepted steam sterilization challenge organism, ≥1.0 × 106 CFU per vial (typical 1.0–1.2 × 106), verified per ISO 11138-1.
📋 Batch Traceability
Every pack carries lot-level traceability. Batch D-value and spore-count documentation available — ask us for the certificate of analysis for your lot.
🇨🇦 Canadian Import & Distribution
Imported and distributed by CliniEco Medical (MDEL #35334) — with English/French labeling and Toronto-based support.
📦 Importer & Regulatory Information
Imported and distributed by: CliniEco Medical (Ecology Source New Energy Technology Co., Ltd.)
255 Duncan Mill Road, Suite 512, Toronto, ON M3B 3H9, Canada
Storage: 15–30°C, away from direct sunlight and moisture. Shelf life: 24 months from manufacture; EXP printed on every vial. For professional use only. Do not use if damaged or expired.
Conforms to EN ISO 11138-3 (moist heat) and EN ISO 11138-1 — third-party test report XDX522601161208BST (BST Testing, EN ISO 11138-1:2017 and -3:2017) available on request. For steam sterilization only — not for dry heat, chemical vapour, ethylene oxide (EO) or other low-temperature processes.
When the 100-Pack Is the Right Box
Each test day uses two indicators from the same lot: one vial goes through the cycle, and the second is the unexposed control that has to turn yellow for the run to count as valid. So the yearly quantity is the number of days the sterilizer runs, doubled.
Indicators a year = test days a year x 2. When that number reaches 100, the 100-pack is the cheaper box to hold.
| Test days a year | Indicators a year | 100-packs a year | Cost in 100-packs, $189 each | The same year bought as 25-packs, $99 each |
| 50 | 100 | 1 | $189.00 | 4 boxes — $396.00 |
| 100 | 200 | 2 | $378.00 | 8 boxes — $792.00 |
| 150 | 300 | 3 | $567.00 | 12 boxes — $1,188.00 |
| 200 | 400 | 4 | $756.00 | 16 boxes — $1,584.00 |
| 240 — one sterilizer, five days a week, 48 weeks | 480 | 5 | $945.00 | 20 boxes — $1,980.00 |
Per test this is the lowest-cost box in the 24-hour line, because a hundred vials spread the box amount furthest. The 25-pack and the 50-pack are priced for smaller order quantities, not for a full working year.
Signs the 100-pack is your size
- One sterilizer, in use more than four days a week, all year.
- Two or more sterilizers on the same bench, each tested on the days it runs — the test days add up faster than the chair count suggests.
- You already reorder the 25-pack three or four times a year; four reorders cost $396 for the same 100 indicators this box carries.
- Multi-site or group purchasing: one delivery, one lot number, one invoice for the year.
Signs you should not buy the 100-pack yet
- Fewer than 50 test days a year, which is under two test days a week: the 50-pack matches that pace and keeps less money on the shelf.
- You are changing brand, cycle type or incubator: start on the 25-pack until one lot reads clean in your own unit, then step up. Buying a year of vials around an unproven routine is the expensive way to learn.
- Check the lot date printed on the box before committing to a year: a 100-pack is only a saving if the expiry covers the calendar you are planning.
How the box paces out
At five test days a week, 100 indicators is about ten weeks of cover — a little over a quarter. At one test day a week it is close to a full year, which makes this the box for a low-frequency practice that would rather order once. Divide 100 by your own test days a week and you get the number of weeks before the next order; if that number is under eight, move up rather than reorder mid-quarter.
The same year, priced against the 50-pack
| Test days a year | Indicators | Bought as 100-packs | Bought as 50-packs, $109.99 each | Difference |
| 100 | 200 | 2 boxes — $378.00 | 4 boxes — $439.96 | $61.96 less |
| 240 | 480 | 5 boxes — $945.00 | 10 boxes — $1,099.90 | $154.90 less |
What the box does not include: the incubator, which is sold separately and is not needed per box, and the record sheet, which is a free printable. The two indicators used per test day both come out of this count, so budget the control vial in the same line as the test vial.
How to Read Your 24-Hour Result
| Negative — pass | The medium stays purple after 24 hours at 56–60 °C (58 ± 2 °C): no spore growth. Release the load. |
| Positive — fail | The medium turns yellow: spores survived the cycle. Re-sterilize everything in that load and re-test before releasing instruments. |
| Incubation | 56–60 °C (58 ± 2 °C) dry-block incubator; read at 24 hours (this indicator is rated for a 24-hour readout, not 48 hours). |
| Positive control | Run one unexposed indicator from the same lot as a control on each day you test; it must turn yellow. If the control does not grow, the test run is not valid. |
| Reading is visual | No reader or UV lamp is needed. |
| Source | Manufacturer's instructions for use supplied with each lot. |
Product Specifications
| Brand | CliniEco Medical |
| Organism | Geobacillus stearothermophilus ATCC 7953 — ≥1.0 × 106 CFU per vial (typical 1.0–1.2 × 106) |
| Readout | 24-hour rapid visual readout (color change) |
| Cycles | 121°C gravity & 132–135°C vacuum-assisted steam sterilization |
| Format | Self-contained vial with growth medium — no transfer steps |
| Pack Size | 100 indicators per pack (2 x 50-count boxes); monitoring log template included |
| Frequency | Daily per sterilizer in Ontario (RCDSO); at least weekly in most US states (CDC / AAMI ST79) |
| D-value / Z-value | Reported for every lot on the CoA — current lot: 2.0 min at 121 °C, Z-value 11.3 °C |
| Distribution | CliniEco MDEL #35334 |
Sample CoA from a production lot — the certificate issued with your shipment carries your lot number and the manufacturer's details.
Features
- ✓ G. stearothermophilus ATCC 7953 — the accepted steam sterilization challenge organism
- ✓ 24-hour result — results a day sooner than 48-hour vials
- ✓ Self-contained (SCBI) — ampoule and medium in one vial
- ✓ Monitoring record log template included — supports RCDSO daily documentation and CDC / AAMI ST79
Compliance
- RCDSO (Ontario): a biological indicator for each sterilizer on every day that it is used — daily, per RCDSO IPAC standard 03/24_5542
- CDC and AAMI ST79: spore test at least weekly per sterilizer (US baseline)
- Test organism: Geobacillus stearothermophilus ATCC 7953 (steam)
- Distributed by CliniEco Medical under MDEL #35334
- Manufactured to EN ISO 11138-3 and EN ISO 11138-1 — documentation available on request
Hardware is sold separately — buy only what you need
We do not bundle hardware into a plan. Prices:
- 24-Hour Dry-Block Incubator — $379
- Rapid Reader, 4-well fluorescence — $1,499 (3-hour results)
Both include free printable record sheets. No lock-in — our BIs work in any standard 56–60 °C (58 ± 2 °C) incubator.
Start BI Plan — Reserve Your Free Device →CliniEco Service Promise
- Free shipping on this 100-pack — anywhere in Canada
- Ships from our next production lot — dispatch date confirmed by email within one business day
- Canadian-owned company
- Batch documentation available — CoA, D-value and spore count on request
Clinic & Volume Pricing
Contact us for volume pricing — email alina@clinieco.ca.
- Volume pricing for clinics, dental practices, LTC homes and distributors
- Free shipping on this item — anywhere in Canada
Why Buy CliniEco?
- 24-hour results, with the per-test figure shown on this page — below the $1.90–$2.35 per test range for comparable 100-count 24-hour indicators from other Canadian suppliers (checked Oct 2026)
- Compliance built in — the monitoring record log template supports RCDSO daily documentation and CDC / AAMI ST79 expectations
- Complete reprocessing line — pairs with CliniEco pouches, rolls, tape and cleaning solution
- Full traceability — factory documentation, batch CoA, Canadian import
Related Reading
Related Guides
Learn how to choose and use this product correctly:
- Sterility Assurance Level (SAL) Explained: What 10-6 Means for Sterilization
- Biological Indicator Failure: Recall, Root Cause, and Re-Test Protocol
- Class 4 vs 5 vs 6 Sterilization Indicators: Which Do You Need?
- How Often Should You Run a Spore Test? Sterilizer Monitoring Schedules
- Free Sterilization Log Sheet Generator — printable, no login
Have a question about your own facility? Send it in and you will get a written answer specific to your setup, with the regulation or standard it is based on cited. Ask your compliance question.
Related tests in this BI line
- Clinic Starter Bundle — Gloves, Spore Testing & Sterilization Packaging
- CliniEco 24-Hour Biological Indicator Incubator — Precision Dry-Block, 24 Wells
- CliniEco Medical Biological Indicator — 1-Hour Rapid Readout, Fluorescence, 50-Pack
- CliniEco Medical Biological Indicator — 20-Minute Rapid Readout, Fluorescence, 50-Pack
- CliniEco Medical Biological Indicator — 24-Hour Self-Contained, 25-Pack
- CliniEco Medical Biological Indicator — 24-Hour Self-Contained, 50-Pack
- CliniEco Medical Biological Indicator — 3-Hour Rapid Readout, Fluorescence, 50-Pack
- Free Biological Indicator 5-Pack (clinics)
- CliniEco Medical Bowie-Dick Test Pack — ISO 11140-5 Type 2, Daily Vacuum Check, 6-Pack
- CliniEco Rapid Reader — 4-Well Fluorescence Biological Indicator Reader
Related articles
- How Often Must US vs Canadian Clinics Run Sterilizer Testing? Which Biological Indicator Pack to Order
- What Is a Biological Indicator? Definition, Core Organism, and Steam Autoclave Formats
- ISO 11138-5 Low-Temperature Steam Formaldehyde Indicators: A Canadian Buyer's Guide
- Biological Indicator Price in Canada: What a Year of Daily Spore Testing Costs a Dental Clinic
Common questions
How often should we run a spore test?
In Ontario, the RCDSO requires a biological indicator for each sterilizer on every day it is used — daily monitoring, not weekly. The weekly baseline is the CDC / AAMI ST79 standard in most US states. A 100-pack covers about 50 monitoring days (one test plus one control each day the sterilizer runs) or about a year of weekly testing.
How do I read the result?
After the cycle, activate the vial per the included instructions and incubate; read at 24 hours. No color change means no surviving spores (pass); a color change means failure — quarantine the load and re-test the sterilizer.
What organism is used, and why?
Geobacillus stearothermophilus ATCC 7953 — the standard biological challenge for steam because of its high heat resistance. A compact dry-block incubator is available on request.
Can I see the factory documentation?
Yes — we provide the test reports behind the standards listed above and batch certificates of analysis on request. Email alina@clinieco.ca.
Does it work with my autoclave cycle type?
This item is used with standard steam sterilization cycles; confirm the specific cycle against the manufacturer's instructions supplied with each lot.
How should it be stored, and how long does it keep?
Store between 15–30 °C at 30–65% relative humidity, keep away from sterilants, direct sunlight and UV, and do not crush the packaging. Shelf life is 24 months from the date of manufacture.
How often does a Canadian clinic need to run it?
In Ontario, the RCDSO requires a biological indicator for each sterilizer on every day that it is used — daily monitoring, not week.
Which standard does it meet?
Manufactured and tested to EN ISO 11138-1 and EN ISO 11138-3.
How long does a 24-hour readout biological indicator take to incubate?
This is a self-contained 24-hour test: after the cycle the indicator is activated and incubated, and the readout is available at the time stated in the manufacturer's instructions for use — around 24 hours. Keep the activated indicator in the incubator for the full period before reading it, and read the result against the control.
Is a 24-hour biological indicator acceptable for RCDSO daily monitoring?
The requirement is about how often you test, not how fast the indicator reads. In Ontario the RCDSO expects a biological indicator for each sterilizer on every day the sterilizer is used — daily monitoring, not weekly — placed in a process challenge device and run for each type of cycle used that day. The weekly interval is the CDC / AAMI ST79 baseline in most US states. A 24-hour readout fits a daily schedule; where same-shift release is needed, a rapid-readout indicator is used instead.
How is this different from the 3-hour indicator and the 5-pack trial?
All three are biological indicators with the same pass-or-fail meaning; the difference is the readout time and the pack size. The 3-hour unit is a fluorescence test for same-shift release, this 24-hour unit suits a next-day schedule, and the 5-pack trial is a small pack to evaluate the routine before committing to a working size.
How many indicators does one test need, and how long should records be kept?
One indicator per sterilizer for each day it is used, plus a control. File the result in a log that matches your facility's records-retention policy, and note the sterilizer, the cycle type and the date alongside it.
